BACKGROUND A positive Trypanosoma cruzi polymerase chain reaction (PCR) is associated with a worse prognosis in patients with chronic Chagas disease (CD). OBJECTIVES To study the association of clinical, electrocardiographic, and echocardiographic characteristics and biomarker blood levels with positive T. cruzi PCR in chronic CD. METHODS This is a single-centre observational cross-sectional study. Positive T. cruzi PCR association with clinical, electrocardiographic, and echocardiographic characteristics, and biomarker blood levels were studied by logistic regression analysis. p values < 0.05 were considered significant. FINDINGS Among 333 patients with chronic CD (56.4% men; 62 ± 10 years), T. cruzi PCR was positive in 41.1%. Stepwise multivariate logistic regression showed an independent association between positive T. cruzi PCR and diabetes mellitus {odds ratio (OR) 0.53 [95% confidence interval (CI) 0.30-0.93]; p = 0.03}, right bundle branch block [OR 1.78 (95% CI 1.09-2.89); p = 0.02], and history of trypanocidal treatment [OR 0.13 (95% CI 0.04-0.38); p = 0.0002]. Among patients with a history of trypanocidal treatment (n = 39), only four (10%) patients had a positive T. cruzi PCR. MAIN CONCLUSIONS Among several studied parameters, only diabetes mellitus, right bundle branch block, and history of trypanocidal treatment showed an independent association with positive T. cruzi PCR. History of trypanocidal treatment was a strong protective factor against a positive T. cruzi PCR.

Resumo Fundamentos: Novas técnicas ecocardiográficas são utilizadas no diagnóstico e prognóstico de diversas cardiopatias. No entanto, muitos desses novos índices ainda carecem de valores de referência em diferentes populações. Estudamos esses novos parâmetros ecocardiográficos em um grupo de brasileiros sem doença cardiovascular conhecida. Objetivo: Estudar valores dos novos índices ecocardiográficos em brasileiros sem doença cardiovascular conhecida e sua correlação com a idade. Métodos: Estudo transversal composto por indivíduos saudáveis que realizaram ecocardiograma tridimensional (E3D) e ecocardiograma bidimensional com análise de deformação (e) por speckle tracking (EST). Foram analisadas as funções atrial esquerda (AE) e ventricular esquerda (VE) por E3D e EST, e a função ventricular direita (VD) por EST. Valores de p < 0,05 foram considerados significantes. Resultados: Foram incluídos setenta e sete indivíduos (46,7% homens; 40,4 ± 10,4 anos). Os volumes AE máximo, mínimo e pré-contração atrial (pré-A) (ml/m2) foram 21,2 ± 5,5, 7,8 ± 2,5 e 11,0 ± 3,1, respectivamente. O pico da e global positiva do AE (LAScd), pico da e global negativa do AE e e global total do AE (LASr) foram 17,4±5,2%, -13,2 ± 2,0% e 30,5 ± 5,9%, respectivamente. Os volumes diastólico final e sistólico final do VE (ml/m2) mediram 57 ± 12 e 24 ± 6 e a fração de ejeção tridimensional do VE mediu 58 ± 6%. A e longitudinal, circunferencial e radial global do VE foi de -19 ± 2%, -19 ± 3% e 46 ± 12%, respectivamente. A torção do VE mediu 1,6 ± 0,7(0)/cm. A e longitudinal global do VD (SLG-VD) e a deformação da parede livre do VD foram de -22 ± 3% e -24 ± 5%. Os volumes mínimo e pré-A do AE, rotação apical do VE, torção e SLG-VD aumentaram com a idade, enquanto as frações de esvaziamento total e passivo do LA, LAScd, LASr, volumes diastólico final e sistólico final do VE diminuíram com a idade. Conclusão: Apresentam-se os valores para os novos índices ecocardiográficos em brasileiros sem doença cardiovascular conhecida e sua correlação com a idade.

Abstract Background: New echocardiographic techniques are used in the diagnosis and prognosis of many heart diseases. However, reference values in different populations are still needed for several of these new indexes. We studied these new echocardiographic parameters in a group of Brazilians with no known cardiovascular disease. Objective: To study values for new echocardiographic indexes in Brazilians without known cardiovascular disease and their correlation with age. Methods: Cross-sectional study that included healthy individuals who underwent three-dimensional echocardiography (3DE) and two-dimensional speckle tracking echocardiography (STE) strain (e) analysis. Left atrial (LA) and left ventricular (LV) function were analyzed by 3DE and STE, and right ventricular (RV) function by STE. P values < 0.05 were considered significant. Results: Seventy-seven subjects (46.7% men; 40.4 ± 10.4 years) were included. Maximum, minimum and pre-atrial contraction (pre-A) LA volumes (ml/m2) were 21.2 ± 5.5, 7.8 ± 2.5, and 11.0 ± 3.1, respectively. Peak positive global LA e (LAScd), peak negative global LA e and total global LA e (LASr) were 17.4 ± 5.2%, -13.2 ± 2.0% and 30.5 ± 5.9%, respectively. LV end-diastolic and end-systolic volumes (ml/m2) measured 57 ± 12 and 24 ± 6, and 3D LV ejection fraction measured 58 ± 6%. Global LV longitudinal, circumferential and radial e were -19 ± 2%, -19 ± 3%, and 46 ± 12%, respectively. LV torsion measured 1.6 ± 0.70 /cm. Global longitudinal RV e (RV-GLS) and RV free wall strain were -22 ± 3% and -24 ± 5%. Minimum LA and pre-A volumes, LV apical rotation, torsion and RV-GLS increased with age, while total and passive LA emptying fractions, LAScd, LASr, LV end-diastolic and end-systolic volumes decreased with age. Conclusion: Values for new echocardiographic indexes in Brazilians without known cardiovascular disease and their correlation with age are presented.

ABSTRACT The use of saline water in agriculture has increased in the Brazilian semiarid due to low availability of good quality water for irrigated agriculture, being the selection of salt tolerant hybrids an alternative to reduce the effect of salinity on nutritional status of plants. So, the objective of this work was to evaluate the effect of salinity of irrigation water in the accumulation and partition of nutrients in the vegetable tissue of watermelon cultivars, in the region of Mossoro in the State of Rio Grande do Norte, Brazil. The treatments studied consisted of applying irrigation water with five electrical conductivities (EC1 = 0.57, EC2 = 1.36, EC3 = 2.77, EC4 = 3.86 and EC5 = 4.91 dS m-1), two watermelon cultivars and four samplings times (15; 29; 43 and 60 days after transplanting), arranged in a scheme of split plot(5x2x4) and outlined in complete blocksrandomized with four replications. The accumulated nutrients in plants were influenced by ECw, occurring a loss in comparison with the higher ECw of 24.1 and 36.4% in the accumulationof N and P in the vegetative part of plant and 37.8 and 30.1 % in the accumulation of P and K in the aerial part. The accumulation of N, P and K in the watermelon was influenced by plant age, with reduction at the end of the cycle.

O uso de águas salinas na agricultura é um dos principais fatores que limitam a produção e a qualidade dos frutos no Estado do Rio Grande do Norte, sendo a seleção de híbridos tolerantes à salinidade e a utilização de adubação nitrogenada, alternativas para se evitar perdas de rendimento e qualidade desses frutos. Assim, o objetivo com este trabalho foi avaliar o comportamento produtivo e a qualidade dos frutos de três cultivares de melancia (Quetzali, Shadow e Leopard) submetidas a diferentes salinidades da água de irrigação e doses de nitrogênio, na região de Mossoró, RN. Os tratamentos consistiram em aplicação de água de irrigação com cinco condutividades elétricas (0,57; 1,36; 2,77; 3,86 e 4,91 dS m-1) e três doses de nitrogênio (48, 96 e 144 kg ha-1), delineados em blocos casualizados, com quatro repetições. Foram avaliados a produtividade, o número e o peso médio dos frutos e o teor de sólidos solúveis. O peso médio e o número de frutos influenciaram as perdas de rendimento das cultivares de melancia estudadas; apenas a cultivar Shadow apresentou o teor de sólidos solúveis dos frutos alterado com a salinidade da água de irrigação.

The use of saline waters in the agriculture is one of the main factors that limit the production and the fruit quality in the State of Rio Grande do Norte, being the selection of tolerant hybrids to salinity and the use of the nitrogen fertilization, alternatives to avoid losses in income and quality of the fruits. Therefore, the objective of this study was to evaluate the production and the quality of the fruits of three cultivars of watermelon (Quetzali, Shadow and Leopard) under different salinities of irrigation water and doses of nitrogen in the region of Mossoró, RN. The treatments consisted in the application of irrigation water with five electrical conductivities (0.57; 1.36; 2.77; 3.86 e 4.91 dS m-1) and three doses of nitrogen (48, 96 e 144 kg ha-1), delineated in randomized blocks, with four replications. The productivity, the number and the mean weight of fruits, as well as the content of soluble solids were evaluated. The mean weight of fruits and the number of fruits affected the loss of income of studied watermelons cultivars. Only the Shadow cultivar had the soluble solids content of fruits altered with the salinity of irrigation water.